Friday, January 22, 2016

pGLO Lab







Two new traits in the bacteria consist of glowing green colonies and ampicillan.

The amount of bacteria that would be in -pGLO LB would be none. The amount for bacteria that would appear in -pGLO LB/Amp is none. The last to are different though as +pGLO LB/Amp would produce around 448 colonies and +pGLO LB/Amp/ara would produce 640 colonies.

The role that the arabinose played was that it attaches to the promoter and then triggers the glowing.


GFP is used in mice. They tried it in mice to test if they can get the florescent look they get in jellyfish an found that the mice glowed everywhere but its hair. They also tried it in plants to test if they were freezer resistant, if the plant glows you know that the plant is freezer resistant. They have even made fish with GFP in it. These fish glow and are actually sold to people. The fact that the fish glows will appeal more to a certain demographic, kids.

An example of another application of genetic engineering would be to figure out if people are related. So for example you can take their genetics and compare them to each other.






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